In low-percentage gels (< 10 ), the low-molecular weight proteins in the ladder may migrate with the dye front. See the table provided for migration patterns in different electrophoresis conditions. Transfer the desired amount of the Prestained Protein Ladder to a separate tube. Precision Plus Protein Unstained Standards are also an excellent choice for use with stain-free gels and theĪll Precision Plus Protein Standards generate a standard curve with r 2 values >0.99, demonstrating a strong linear relationship between the proteins' log molecular weight and relative migration distance on a gel. against unstained standards in the same system. Vortex gently to ensure the solution is homogeneous. These protein standards are compatible with Laemmli and neutral pH buffer systems and can be used for accurate molecular weight determination on gels and blots. These standards offer absolute molecular weight accuracy confirmed by mass spectrometry, with a known amount of protein in each band to allow approximation of protein concentration in your sample. Precision Plus Protein Unstained Standards contain the unique Strep-tag affinity peptide, which allows detection and molecular weight determination on western blots. These unstained protein standards include three high-intensity reference bands (25, 50, and 75 kD). These standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD. Precision Plus Protein™ Standards for protein electrophoresis offer unsurpassed band sharpness, accurate MW determination, lot-to-lot consistency, and excellent blotting results. (Unstained protein ladders have only the bromophenol blue at the dye front to help monitor progress it does not help to assess blotting efficiency.Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. 26634 Spectra Multicolor Broad Range Protein Ladder, 2 × 250µL. 26632 PageRuler Low Range Unstained Protein Ladder, 2 × 250µL. 26630 PageRuler Broad Range Unstained Protein Ladder, 2 × 250µL. They offer easier identification of protein molecular weights and the ability to monitor migration during electrophoresis. 26619 PageRuler Plus Prestained Protein Ladder, 2 × 250µL. These prestained recombinant protein ladders include three high-intensity reference bands: 25, 50, and 75 kD. Calculate the approximate size of your protein by comparing the image of the membrane with the ladder to the image produced by antibody staining 5 l of 10-180kDa Prestained Protein Ladder resolves 10 bands in 4-20 SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.Verify transfer efficiency between the gel and PVDF, nylon, or nitrocellulose membranes.Monitor protein separation during SDS-polyacrylamide gel electrophoresis This unstained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer.In western blotting, prestained protein ladders can be used to: Each protein can be stained with a different color for easy identification or labeled with affinity tags for western blot detection. Unstained Protein Ladders MagicMark XP Western Protein Standard: 20220: 9: all SDS-PAGE gels: stain of choice : IgG binding sites: PageRuler Unstained Protein Ladder: 10200: 14: all SDS-PAGE gels: stain of choice : Strep-tag II sequence : PageRuler Unstained Broad Range Protein Ladder: 5250: 11: all SDS-PAGE gels: stain of choice. Recombinant protein ladders are engineered to produce tight bands, evenly spaced molecular weights, and other specific traits. Although some laboratories continue to use natural protein ladders, recombinant standards are more commonly used. PI62299), which is gentle and designed for quickly removing primary and near-infrared (IR) dyelabeled secondary antibodies from western blots. Fluorescent Western Blot Stripping Buffer (Cat. These broad bands are less distinct, which makes them less useful for molecular weight estimation. Want to detect PageRuler unstained protein ladders in your chemiluminescence blot. Western blot analysis of 59329 using Anti-Blue (2DF11) Mouse mAb (HRP. Prestained natural proteins may produce broader bands than recombinant proteins, especially when they are stained with more than one color. Titration of Blue Prestained Protein Marker, Broad Range (11-250 kDa) 59329 (left). The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Prestained naturally occurring proteins will vary due to the amount and location of dye binding sites. Low price but you get a very good quality of protein ladder Applications: Western Blot,Protein purification. HiMark Unstained High Molecular Weight (HMW) Protein Standard contains nine proteins in the range of 40500 kDa designed for analysis of high molecular weight proteins.
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